{"id":1645,"date":"2022-07-15T12:49:13","date_gmt":"2022-07-15T12:49:13","guid":{"rendered":"http:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/?page_id=1645"},"modified":"2023-03-08T10:51:28","modified_gmt":"2023-03-08T10:51:28","slug":"ht2_l1-fundamentals-of-macromolecular-crystallography","status":"publish","type":"page","link":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/ht2_l1-fundamentals-of-macromolecular-crystallography\/","title":{"rendered":"HT2 L1: Fundamentals of macromolecular crystallography"},"content":{"rendered":"<p><strong>HT2: Processing<img loading=\"lazy\" decoding=\"async\" class=\"wp-image-1158 alignright\" src=\"http:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-content\/uploads\/2022\/06\/2021-03-24-09.39.15-scaled-e1655748676200-300x300.jpg\" alt=\"\" width=\"153\" height=\"153\" srcset=\"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-content\/uploads\/2022\/06\/2021-03-24-09.39.15-scaled-e1655748676200-300x300.jpg 300w, https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-content\/uploads\/2022\/06\/2021-03-24-09.39.15-scaled-e1655748676200-1024x1024.jpg 1024w, https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-content\/uploads\/2022\/06\/2021-03-24-09.39.15-scaled-e1655748676200-150x150.jpg 150w, https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-content\/uploads\/2022\/06\/2021-03-24-09.39.15-scaled-e1655748676200-768x768.jpg 768w, https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-content\/uploads\/2022\/06\/2021-03-24-09.39.15-scaled-e1655748676200-400x400.jpg 400w, https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-content\/uploads\/2022\/06\/2021-03-24-09.39.15-scaled-e1655748676200-100x100.jpg 100w, https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-content\/uploads\/2022\/06\/2021-03-24-09.39.15-scaled-e1655748676200.jpg 1452w\" sizes=\"auto, (max-width: 153px) 100vw, 153px\" \/><\/strong><\/p>\n<h4>L1 (Lecture): Fundamentals of Macromolecular Crystallography<\/h4>\n<p><strong>Edward Lowe<\/strong><br \/>\nDepartment of Biochemistry<br \/>\nUniversity of Oxford<br \/>\nOxford, UK<\/p>\n<p><em>Crystal -&gt; Space groups -&gt; Diffraction -&gt; Phase problem -&gt; Model building -&gt; Refinement<\/em><\/p>\n<p>Protein crystallography is a powerful technique for the determination of the structures of biological macromolecules at atomic or near-atomic resolution. Once crystals of a protein of interest have been obtained and harvested the X-ray crystallography experiment consists of a number of stages, each of which has a substantial theoretical underpinning. This lecture will briefly introduce each of these stages along with their underlying theory and will aim to set the stage for more detailed treatments of several of the featured subjects later in the conference. We will introduce the concepts of diffraction, symmetry and the phase problem and will briefly discuss some of the methods used to collect diffraction data, solve the phase problem and build molecular models.<\/p>\n<p>Recommended Reading:<\/p>\n<p>Rupp, B. <strong>Biomolecular Crystallography: Principles, Practice, and Application to Structural Biology<\/strong> 2009. Garland Science. 809 pages<\/p>\n","protected":false},"excerpt":{"rendered":"<p>HT2: Processing L1 (Lecture): Fundamentals of Macromolecular Crystallography Edward Lowe Department of Biochemistry University of Oxford Oxford, UK Crystal -&gt; Space groups -&gt; Diffraction -&gt; Phase problem -&gt; Model building -&gt; Refinement Protein crystallography is a powerful technique for the determination of the structures of biological macromolecules at atomic or near-atomic resolution. Once crystals of [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"ngg_post_thumbnail":0,"footnotes":""},"class_list":["post-1645","page","type-page","status-publish","hentry"],"aioseo_notices":[],"aioseo_head":"\n\t\t<!-- All in One SEO 5.0.2.1 - aioseo.com -->\n\t<meta name=\"description\" content=\"HT2: Processing L1 (Lecture): Fundamentals of Macromolecular Crystallography Edward Lowe Department of Biochemistry University of Oxford Oxford, UK Crystal -&gt; Space groups -&gt; Diffraction -&gt; Phase problem -&gt; Model building -&gt; Refinement Protein crystallography is a powerful technique for the determination of the structures of biological macromolecules at atomic or near-atomic resolution. Once crystals of\" \/>\n\t<meta name=\"robots\" content=\"max-image-preview:large\" \/>\n\t<link rel=\"canonical\" href=\"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/ht2_l1-fundamentals-of-macromolecular-crystallography\/\" \/>\n\t<meta name=\"generator\" content=\"All in One SEO (AIOSEO) 5.0.2.1\" \/>\n\t\t<meta property=\"og:locale\" content=\"en_US\" \/>\n\t\t<meta property=\"og:site_name\" content=\"HTCC5 - HOT TOPICS IN CONTEMPORARY CRYSTALLOGRAPHY 5\" \/>\n\t\t<meta property=\"og:type\" content=\"article\" \/>\n\t\t<meta property=\"og:title\" content=\"HT2 L1: Fundamentals of macromolecular crystallography - HTCC5\" \/>\n\t\t<meta property=\"og:description\" content=\"HT2: Processing L1 (Lecture): Fundamentals of Macromolecular Crystallography Edward Lowe Department of Biochemistry University of Oxford Oxford, UK Crystal -&gt; Space groups -&gt; Diffraction -&gt; Phase problem -&gt; Model building -&gt; Refinement Protein crystallography is a powerful technique for the determination of the structures of biological macromolecules at atomic or near-atomic resolution. Once crystals of\" \/>\n\t\t<meta property=\"og:url\" content=\"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/ht2_l1-fundamentals-of-macromolecular-crystallography\/\" \/>\n\t\t<meta property=\"og:image\" content=\"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-content\/uploads\/2022\/06\/cropped-cropped-HTCC5_logo_mali_2.jpg\" \/>\n\t\t<meta property=\"og:image:secure_url\" content=\"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-content\/uploads\/2022\/06\/cropped-cropped-HTCC5_logo_mali_2.jpg\" \/>\n\t\t<meta property=\"article:published_time\" content=\"2022-07-15T12:49:13+00:00\" \/>\n\t\t<meta property=\"article:modified_time\" content=\"2023-03-08T10:51:28+00:00\" \/>\n\t\t<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n\t\t<meta name=\"twitter:title\" content=\"HT2 L1: Fundamentals of macromolecular crystallography - HTCC5\" \/>\n\t\t<meta name=\"twitter:description\" content=\"HT2: Processing L1 (Lecture): Fundamentals of Macromolecular Crystallography Edward Lowe Department of Biochemistry University of Oxford Oxford, UK Crystal -&gt; Space groups -&gt; Diffraction -&gt; Phase problem -&gt; Model building -&gt; Refinement Protein crystallography is a powerful technique for the determination of the structures of biological macromolecules at atomic or near-atomic resolution. Once crystals of\" \/>\n\t\t<meta name=\"twitter:image\" content=\"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-content\/uploads\/2022\/06\/cropped-cropped-HTCC5_logo_mali_2.jpg\" \/>\n\t\t<script type=\"application\/ld+json\" class=\"aioseo-schema\">\n\t\t\t{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/hrvatska-udruga-kristalografa.hr\\\/htcc5\\\/ht2_l1-fundamentals-of-macromolecular-crystallography\\\/#breadcrumblist\",\"itemListElement\":[{\"@type\":\"ListItem\",\"@id\":\"https:\\\/\\\/hrvatska-udruga-kristalografa.hr\\\/htcc5\\\/#listItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/hrvatska-udruga-kristalografa.hr\\\/htcc5\\\/\",\"nextItem\":{\"@type\":\"ListItem\",\"@id\":\"https:\\\/\\\/hrvatska-udruga-kristalografa.hr\\\/htcc5\\\/ht2_l1-fundamentals-of-macromolecular-crystallography\\\/#listItem\",\"name\":\"HT2 L1: Fundamentals of macromolecular crystallography\"}},{\"@type\":\"ListItem\",\"@id\":\"https:\\\/\\\/hrvatska-udruga-kristalografa.hr\\\/htcc5\\\/ht2_l1-fundamentals-of-macromolecular-crystallography\\\/#listItem\",\"position\":2,\"name\":\"HT2 L1: Fundamentals of macromolecular crystallography\",\"previousItem\":{\"@type\":\"ListItem\",\"@id\":\"https:\\\/\\\/hrvatska-udruga-kristalografa.hr\\\/htcc5\\\/#listItem\",\"name\":\"Home\"}}]},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/hrvatska-udruga-kristalografa.hr\\\/htcc5\\\/#organization\",\"name\":\"HTCC5\",\"description\":\"HOT TOPICS IN CONTEMPORARY CRYSTALLOGRAPHY 5\",\"url\":\"https:\\\/\\\/hrvatska-udruga-kristalografa.hr\\\/htcc5\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"url\":\"http:\\\/\\\/hrvatska-udruga-kristalografa.hr\\\/wp-content\\\/uploads\\\/2022\\\/06\\\/cropped-Untitled-1.jpg\",\"@id\":\"https:\\\/\\\/hrvatska-udruga-kristalografa.hr\\\/htcc5\\\/ht2_l1-fundamentals-of-macromolecular-crystallography\\\/#organizationLogo\"},\"image\":{\"@id\":\"https:\\\/\\\/hrvatska-udruga-kristalografa.hr\\\/htcc5\\\/ht2_l1-fundamentals-of-macromolecular-crystallography\\\/#organizationLogo\"}},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/hrvatska-udruga-kristalografa.hr\\\/htcc5\\\/ht2_l1-fundamentals-of-macromolecular-crystallography\\\/#webpage\",\"url\":\"https:\\\/\\\/hrvatska-udruga-kristalografa.hr\\\/htcc5\\\/ht2_l1-fundamentals-of-macromolecular-crystallography\\\/\",\"name\":\"HT2 L1: Fundamentals of macromolecular crystallography - HTCC5\",\"description\":\"HT2: Processing L1 (Lecture): Fundamentals of Macromolecular Crystallography Edward Lowe Department of Biochemistry University of Oxford Oxford, UK Crystal -> Space groups -> Diffraction -> Phase problem -> Model building -> Refinement Protein crystallography is a powerful technique for the determination of the structures of biological macromolecules at atomic or near-atomic resolution. Once crystals of\",\"inLanguage\":\"en-US\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/hrvatska-udruga-kristalografa.hr\\\/htcc5\\\/#website\"},\"breadcrumb\":{\"@id\":\"https:\\\/\\\/hrvatska-udruga-kristalografa.hr\\\/htcc5\\\/ht2_l1-fundamentals-of-macromolecular-crystallography\\\/#breadcrumblist\"},\"datePublished\":\"2022-07-15T12:49:13+00:00\",\"dateModified\":\"2023-03-08T10:51:28+00:00\"},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/hrvatska-udruga-kristalografa.hr\\\/htcc5\\\/#website\",\"url\":\"https:\\\/\\\/hrvatska-udruga-kristalografa.hr\\\/htcc5\\\/\",\"name\":\"HTCC5\",\"description\":\"HOT TOPICS IN CONTEMPORARY CRYSTALLOGRAPHY 5\",\"inLanguage\":\"en-US\",\"publisher\":{\"@id\":\"https:\\\/\\\/hrvatska-udruga-kristalografa.hr\\\/htcc5\\\/#organization\"}}]}\n\t\t<\/script>\n\t\t<!-- All in One SEO -->\n\n","aioseo_head_json":{"title":"HT2 L1: Fundamentals of macromolecular crystallography - HTCC5","description":"HT2: Processing L1 (Lecture): Fundamentals of Macromolecular Crystallography Edward Lowe Department of Biochemistry University of Oxford Oxford, UK Crystal -> Space groups -> Diffraction -> Phase problem -> Model building -> Refinement Protein crystallography is a powerful technique for the determination of the structures of biological macromolecules at atomic or near-atomic resolution. Once crystals of","canonical_url":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/ht2_l1-fundamentals-of-macromolecular-crystallography\/","robots":"max-image-preview:large","keywords":"","webmasterTools":{"miscellaneous":""},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"BreadcrumbList","@id":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/ht2_l1-fundamentals-of-macromolecular-crystallography\/#breadcrumblist","itemListElement":[{"@type":"ListItem","@id":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/#listItem","position":1,"name":"Home","item":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/","nextItem":{"@type":"ListItem","@id":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/ht2_l1-fundamentals-of-macromolecular-crystallography\/#listItem","name":"HT2 L1: Fundamentals of macromolecular crystallography"}},{"@type":"ListItem","@id":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/ht2_l1-fundamentals-of-macromolecular-crystallography\/#listItem","position":2,"name":"HT2 L1: Fundamentals of macromolecular crystallography","previousItem":{"@type":"ListItem","@id":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/#listItem","name":"Home"}}]},{"@type":"Organization","@id":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/#organization","name":"HTCC5","description":"HOT TOPICS IN CONTEMPORARY CRYSTALLOGRAPHY 5","url":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/","logo":{"@type":"ImageObject","url":"http:\/\/hrvatska-udruga-kristalografa.hr\/wp-content\/uploads\/2022\/06\/cropped-Untitled-1.jpg","@id":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/ht2_l1-fundamentals-of-macromolecular-crystallography\/#organizationLogo"},"image":{"@id":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/ht2_l1-fundamentals-of-macromolecular-crystallography\/#organizationLogo"}},{"@type":"WebPage","@id":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/ht2_l1-fundamentals-of-macromolecular-crystallography\/#webpage","url":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/ht2_l1-fundamentals-of-macromolecular-crystallography\/","name":"HT2 L1: Fundamentals of macromolecular crystallography - HTCC5","description":"HT2: Processing L1 (Lecture): Fundamentals of Macromolecular Crystallography Edward Lowe Department of Biochemistry University of Oxford Oxford, UK Crystal -> Space groups -> Diffraction -> Phase problem -> Model building -> Refinement Protein crystallography is a powerful technique for the determination of the structures of biological macromolecules at atomic or near-atomic resolution. Once crystals of","inLanguage":"en-US","isPartOf":{"@id":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/#website"},"breadcrumb":{"@id":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/ht2_l1-fundamentals-of-macromolecular-crystallography\/#breadcrumblist"},"datePublished":"2022-07-15T12:49:13+00:00","dateModified":"2023-03-08T10:51:28+00:00"},{"@type":"WebSite","@id":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/#website","url":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/","name":"HTCC5","description":"HOT TOPICS IN CONTEMPORARY CRYSTALLOGRAPHY 5","inLanguage":"en-US","publisher":{"@id":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/#organization"}}]},"og:locale":"en_US","og:site_name":"HTCC5 - HOT TOPICS IN CONTEMPORARY CRYSTALLOGRAPHY 5","og:type":"article","og:title":"HT2 L1: Fundamentals of macromolecular crystallography - HTCC5","og:description":"HT2: Processing L1 (Lecture): Fundamentals of Macromolecular Crystallography Edward Lowe Department of Biochemistry University of Oxford Oxford, UK Crystal -&gt; Space groups -&gt; Diffraction -&gt; Phase problem -&gt; Model building -&gt; Refinement Protein crystallography is a powerful technique for the determination of the structures of biological macromolecules at atomic or near-atomic resolution. Once crystals of","og:url":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/ht2_l1-fundamentals-of-macromolecular-crystallography\/","og:image":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-content\/uploads\/2022\/06\/cropped-cropped-HTCC5_logo_mali_2.jpg","og:image:secure_url":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-content\/uploads\/2022\/06\/cropped-cropped-HTCC5_logo_mali_2.jpg","article:published_time":"2022-07-15T12:49:13+00:00","article:modified_time":"2023-03-08T10:51:28+00:00","twitter:card":"summary_large_image","twitter:title":"HT2 L1: Fundamentals of macromolecular crystallography - HTCC5","twitter:description":"HT2: Processing L1 (Lecture): Fundamentals of Macromolecular Crystallography Edward Lowe Department of Biochemistry University of Oxford Oxford, UK Crystal -&gt; Space groups -&gt; Diffraction -&gt; Phase problem -&gt; Model building -&gt; Refinement Protein crystallography is a powerful technique for the determination of the structures of biological macromolecules at atomic or near-atomic resolution. Once crystals of","twitter:image":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-content\/uploads\/2022\/06\/cropped-cropped-HTCC5_logo_mali_2.jpg"},"aioseo_meta_data":{"post_id":"1645","title":null,"description":null,"keywords":[],"keyphrases":{"focus":{"keyphrase":"","score":0,"analysis":{"keyphraseInTitle":{"score":0,"maxScore":9,"error":1}}},"additional":[]},"primary_term":null,"canonical_url":null,"og_title":null,"og_description":null,"og_object_type":"default","og_image_type":"default","og_image_url":null,"og_image_width":null,"og_image_height":null,"og_image_custom_url":null,"og_image_custom_fields":null,"og_video":"","og_custom_url":null,"og_article_section":null,"og_article_tags":[],"twitter_use_og":false,"twitter_card":"default","twitter_image_type":"default","twitter_image_url":null,"twitter_image_custom_url":null,"twitter_image_custom_fields":null,"twitter_title":null,"twitter_description":null,"schema":{"blockGraphs":[],"customGraphs":[],"default":{"data":{"Article":[],"Course":[],"Dataset":[],"FAQPage":[],"Movie":[],"Person":[],"Product":[],"ProductReview":[],"Car":[],"Recipe":[],"Service":[],"SoftwareApplication":[],"WebPage":[]},"graphName":"WebPage","isEnabled":true},"graphs":[]},"schema_type":"default","schema_type_options":null,"pillar_content":false,"robots_default":true,"robots_noindex":false,"robots_noarchive":false,"robots_nosnippet":false,"robots_nofollow":false,"robots_noimageindex":false,"robots_noodp":false,"robots_notranslate":false,"robots_max_snippet":"-1","robots_max_videopreview":"-1","robots_max_imagepreview":"large","priority":null,"frequency":"default","local_seo":null,"limit_modified_date":false,"created":"2022-10-18 08:48:20","updated":"2026-10-05 14:32:45","focus_keyword":null,"additional_keywords":null,"truseo_locale":null,"ai":null,"breadcrumb_settings":null,"seo_analyzer_scan_date":null},"aioseo_breadcrumb":"<div class=\"aioseo-breadcrumbs\"><span class=\"aioseo-breadcrumb\">\n\t\t\t<a href=\"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/\" title=\"Home\">Home<\/a>\n\t\t<\/span><span class=\"aioseo-breadcrumb-separator\">\u00bb<\/span><span class=\"aioseo-breadcrumb\">\n\t\t\tHT2 L1: Fundamentals of macromolecular crystallography\n\t\t<\/span><\/div>","aioseo_breadcrumb_json":[{"label":"Home","link":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/"},{"label":"HT2 L1: Fundamentals of macromolecular crystallography","link":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/ht2_l1-fundamentals-of-macromolecular-crystallography\/"}],"_links":{"self":[{"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-json\/wp\/v2\/pages\/1645","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-json\/wp\/v2\/comments?post=1645"}],"version-history":[{"count":2,"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-json\/wp\/v2\/pages\/1645\/revisions"}],"predecessor-version":[{"id":2416,"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-json\/wp\/v2\/pages\/1645\/revisions\/2416"}],"wp:attachment":[{"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-json\/wp\/v2\/media?parent=1645"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}