{"id":2161,"date":"2022-09-19T07:41:02","date_gmt":"2022-09-19T07:41:02","guid":{"rendered":"http:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/?page_id=2161"},"modified":"2023-04-04T11:11:15","modified_gmt":"2023-04-04T11:11:15","slug":"rmms-microseeding-workshop-participant-questionnaire","status":"publish","type":"page","link":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/rmms-microseeding-workshop-participant-questionnaire\/","title":{"rendered":"A1 bring your sample &#8211; participant questionnaire"},"content":{"rendered":"<p style=\"text-align: justify;\">We look forward to meeting you in&nbsp;Dubrovnik for the&nbsp;HTCC&nbsp;meeting in April. Please bring proteins and a plate containing crystals to participate in the rMMS microseeding workshop. Everything else including plates and screens will be provided.<\/p>\n<p style=\"text-align: justify;\">MMS microseeding can generate new crystals in different conditions. It is ideal if you have crystals that:<\/p>\n<ul style=\"text-align: justify;\">\n<li>Don\u2019t diffract<\/li>\n<li>Are difficult to optimize<\/li>\n<li>Require a different morphology or space group.<\/li>\n<\/ul>\n<p style=\"text-align: justify;\">The technique is also useful if you have crystals of a related protein. Cross seeding is becoming routine and can be used as long as there is some homology between the protein and the seed crystals. Cross-seeding is great for:<\/p>\n<p>Seeding&nbsp;into complexes<\/p>\n<ul style=\"text-align: justify;\">\n<li>Seleno methylamine or heavy atom derivatives<\/li>\n<li>Seeding&nbsp;into Different constructs or species (see second reference below)<\/li>\n<\/ul>\n<p style=\"text-align: justify;\">Students who bring crystals and protein will make a seed stock and set up MMS experiments with MMS workshop tutor Patrick Shaw Stewart. If you are unable to bring crystals and protein, you will still benefit from the workshop and learn about MMS techniques with a test protein.<\/p>\n<p style=\"text-align: justify;\">To help us maximize the chances of good results with MMS please fill out the questionnaire below.<\/p>\n<p style=\"text-align: justify;\">We look forward to seeing you there.<\/p>\n<p style=\"text-align: justify;\">Patrick Shaw Stewart<\/p>\n<p>&nbsp;<\/p>\n<script type=\"text\/javascript\">var gform;gform||(document.addEventListener(\"gform_main_scripts_loaded\",function(){gform.scriptsLoaded=!0}),window.addEventListener(\"DOMContentLoaded\",function(){gform.domLoaded=!0}),gform={domLoaded:!1,scriptsLoaded:!1,initializeOnLoaded:function(o){gform.domLoaded&&gform.scriptsLoaded?o():!gform.domLoaded&&gform.scriptsLoaded?window.addEventListener(\"DOMContentLoaded\",o):document.addEventListener(\"gform_main_scripts_loaded\",o)},hooks:{action:{},filter:{}},addAction:function(o,n,r,t){gform.addHook(\"action\",o,n,r,t)},addFilter:function(o,n,r,t){gform.addHook(\"filter\",o,n,r,t)},doAction:function(o){gform.doHook(\"action\",o,arguments)},applyFilters:function(o){return gform.doHook(\"filter\",o,arguments)},removeAction:function(o,n){gform.removeHook(\"action\",o,n)},removeFilter:function(o,n,r){gform.removeHook(\"filter\",o,n,r)},addHook:function(o,n,r,t,i){null==gform.hooks[o][n]&&(gform.hooks[o][n]=[]);var e=gform.hooks[o][n];null==i&&(i=n+\"_\"+e.length),gform.hooks[o][n].push({tag:i,callable:r,priority:t=null==t?10:t})},doHook:function(n,o,r){var t;if(r=Array.prototype.slice.call(r,1),null!=gform.hooks[n][o]&&((o=gform.hooks[n][o]).sort(function(o,n){return o.priority-n.priority}),o.forEach(function(o){\"function\"!=typeof(t=o.callable)&&(t=window[t]),\"action\"==n?t.apply(null,r):r[0]=t.apply(null,r)})),\"filter\"==n)return r[0]},removeHook:function(o,n,t,i){var r;null!=gform.hooks[o][n]&&(r=(r=gform.hooks[o][n]).filter(function(o,n,r){return!!(null!=i&&i!=o.tag||null!=t&&t!=o.priority)}),gform.hooks[o][n]=r)}});<\/script>\n                <div class='gf_browser_gecko gform_wrapper gravity-theme' id='gform_wrapper_4' >\n                        <div class='gform_heading'>\n                            <h2 class=\"gform_title\">rMMS Microseeding Workshop \u2013 Participant Questionnaire<\/h2>\n                            <span class='gform_description'><\/span>\n                        <\/div><form method='post' enctype='multipart\/form-data'  id='gform_4'  action='\/htcc5\/wp-json\/wp\/v2\/pages\/2161' novalidate>\n                        <div class='gform_body gform-body'><div id='gform_fields_4' class='gform_fields top_label form_sublabel_below description_below'><fieldset id=\"field_4_8\"  class=\"gfield gfield--width-full gfield_contains_required field_sublabel_below field_description_below gfield_visibility_visible\"  data-js-reload=\"field_4_8\"><legend class='gfield_label gfield_label_before_complex'  >Name<span class=\"gfield_required\"><span class=\"gfield_required gfield_required_text\">(Required)<\/span><\/span><\/legend><div class='ginput_complex ginput_container no_prefix has_first_name no_middle_name has_last_name no_suffix gf_name_has_2 ginput_container_name' id='input_4_8'>\n                            \n                            <span id='input_4_8_3_container' class='name_first' >\n                                                    <input type='text' name='input_8.3' id='input_4_8_3' value=''   aria-required='true'     \/>\n                                                    <label for='input_4_8_3' >First<\/label>\n                                                <\/span>\n                            \n                            <span id='input_4_8_6_container' class='name_last' >\n                                                    <input type='text' name='input_8.6' id='input_4_8_6' value=''   aria-required='true'     \/>\n                                                    <label for='input_4_8_6' >Last<\/label>\n                                                <\/span>\n                            \n                        <\/div><\/fieldset><div id=\"field_4_4\"  class=\"gfield gfield--width-full gfield_contains_required field_sublabel_below field_description_below gfield_visibility_visible\"  data-js-reload=\"field_4_4\"><label class='gfield_label' for='input_4_4' >e-mail<span class=\"gfield_required\"><span class=\"gfield_required gfield_required_text\">(Required)<\/span><\/span><\/label><div class='ginput_container ginput_container_text'><input name='input_4' id='input_4_4' type='text' value='' class='large'     aria-required=\"true\" aria-invalid=\"false\"   \/> <\/div><\/div><fieldset id=\"field_4_1\"  class=\"gfield gfield_contains_required field_sublabel_below field_description_below gfield_visibility_visible\"  data-js-reload=\"field_4_1\"><legend class='gfield_label'  >What is the reason to try random MMS?<span class=\"gfield_required\"><span class=\"gfield_required gfield_required_text\">(Required)<\/span><\/span><\/legend><div class='ginput_container ginput_container_radio'><div class='gfield_radio' id='input_4_1'>\n\t\t\t<div class='gchoice gchoice_4_1_0'>\n\t\t\t\t\t<input class='gfield-choice-input' name='input_1' type='radio' value='poor diffraction'  id='choice_4_1_0' onchange='gformToggleRadioOther( this )'    \/>\n\t\t\t\t\t<label for='choice_4_1_0' id='label_4_1_0'>poor diffraction<\/label>\n\t\t\t<\/div>\n\t\t\t<div class='gchoice gchoice_4_1_1'>\n\t\t\t\t\t<input class='gfield-choice-input' name='input_1' type='radio' value='looking for new space group'  id='choice_4_1_1' onchange='gformToggleRadioOther( this )'    \/>\n\t\t\t\t\t<label for='choice_4_1_1' id='label_4_1_1'>looking for new space group<\/label>\n\t\t\t<\/div>\n\t\t\t<div class='gchoice gchoice_4_1_2'>\n\t\t\t\t\t<input class='gfield-choice-input' name='input_1' type='radio' value='want to try cross seeding to new related protein'  id='choice_4_1_2' onchange='gformToggleRadioOther( this )'    \/>\n\t\t\t\t\t<label for='choice_4_1_2' id='label_4_1_2'>want to try cross seeding to new related protein<\/label>\n\t\t\t<\/div><\/div><\/div><\/fieldset><fieldset id=\"field_4_5\"  class=\"gfield gfield--width-full gfield_contains_required field_sublabel_below field_description_below gfield_visibility_visible\"  data-js-reload=\"field_4_5\"><legend class='gfield_label'  >Can the condition be reproduced to make a plate of crystals for seeding?<span class=\"gfield_required\"><span class=\"gfield_required gfield_required_text\">(Required)<\/span><\/span><\/legend><div class='ginput_container ginput_container_radio'><div class='gfield_radio' id='input_4_5'>\n\t\t\t<div class='gchoice gchoice_4_5_0'>\n\t\t\t\t\t<input class='gfield-choice-input' name='input_5' type='radio' value='Yes'  id='choice_4_5_0' onchange='gformToggleRadioOther( this )'    \/>\n\t\t\t\t\t<label for='choice_4_5_0' id='label_4_5_0'>Yes<\/label>\n\t\t\t<\/div>\n\t\t\t<div class='gchoice gchoice_4_5_1'>\n\t\t\t\t\t<input class='gfield-choice-input' name='input_5' type='radio' value='No'  id='choice_4_5_1' onchange='gformToggleRadioOther( this )'    \/>\n\t\t\t\t\t<label for='choice_4_5_1' id='label_4_5_1'>No<\/label>\n\t\t\t<\/div><\/div><\/div><\/fieldset><div id=\"field_4_6\"  class=\"gfield gfield_contains_required field_sublabel_below field_description_below gfield_visibility_visible\"  data-js-reload=\"field_4_6\"><label class='gfield_label' for='input_4_6' >What screening condition(s) were the crystals found in?<span class=\"gfield_required\"><span class=\"gfield_required gfield_required_text\">(Required)<\/span><\/span><\/label><div class='ginput_container ginput_container_textarea'><textarea name='input_6' id='input_4_6' class='textarea large'   maxlength='300'  aria-required=\"true\" aria-invalid=\"false\"   rows='10' cols='50'><\/textarea><\/div><\/div><fieldset id=\"field_4_7\"  class=\"gfield gfield_contains_required field_sublabel_below field_description_below gfield_visibility_visible\"  data-js-reload=\"field_4_7\"><legend class='gfield_label'  >Please provide\/ attach a picture of the potential source crystals for the seed stock.<span class=\"gfield_required\"><span class=\"gfield_required gfield_required_text\">(Required)<\/span><\/span><\/legend><div class='ginput_complex ginput_container ginput_container_post_image'><span class='ginput_full'><input 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<\/div>\n","protected":false},"excerpt":{"rendered":"<p>We look forward to meeting you in&nbsp;Dubrovnik for the&nbsp;HTCC&nbsp;meeting in April. Please bring proteins and a plate containing crystals to participate in the rMMS microseeding workshop. Everything else including plates and screens will be provided. MMS microseeding can generate new crystals in different conditions. It is ideal if you have crystals that: Don\u2019t diffract Are [&hellip;]<\/p>\n","protected":false},"author":5,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"ngg_post_thumbnail":0,"footnotes":""},"class_list":["post-2161","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-json\/wp\/v2\/pages\/2161","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-json\/wp\/v2\/comments?post=2161"}],"version-history":[{"count":8,"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-json\/wp\/v2\/pages\/2161\/revisions"}],"predecessor-version":[{"id":2392,"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-json\/wp\/v2\/pages\/2161\/revisions\/2392"}],"wp:attachment":[{"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-json\/wp\/v2\/media?parent=2161"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}