{"id":2609,"date":"2023-04-04T13:04:03","date_gmt":"2023-04-04T13:04:03","guid":{"rendered":"http:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/?page_id=2609"},"modified":"2023-04-04T13:04:03","modified_gmt":"2023-04-04T13:04:03","slug":"a11-cryogenic-x-ray-crystallographic-studies-of-biomacromolecules-at-turkish-light-source-turkish-delight","status":"publish","type":"page","link":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/abstracts\/a11-cryogenic-x-ray-crystallographic-studies-of-biomacromolecules-at-turkish-light-source-turkish-delight\/","title":{"rendered":"A11: Cryogenic X-ray crystallographic studies of biomacromolecules at Turkish Light Source \u201cTurkish DeLight”"},"content":{"rendered":"

Cahine Kulakman<\/strong>1<\/sup>, Hasan DeMirci1,2<\/sup><\/p>\n

 <\/p>\n

1<\/sup>Department of Molecular Biology and Genetics, Koc University, 34450, Istanbul, Turkey
\n2<\/sup>Stanford PULSE Institute, SLAC National Laboratory, 94025, Menlo Park, CA, USA<\/p>\n

ckulakman22@ku.edu.tr<\/a><\/p>\n

 <\/p>\n

X-ray crystallography is a robust and powerful structural biology technique that provides high-resolution atomic structures of biomacromolecules. Scientists use this technique to unravel mechanistic and structural details of biological macromolecules (e.g. proteins, nucleic acids, protein complexes, protein-nucleic acid complexes, or large biological compartments). Since its inception, single-crystal cryo-crystallography has never been performed in T\u00fcrkiye due to the lack of a single-crystal X-ray diffractometer. The X-ray diffraction facility recently established at the University of Health Sciences, Istanbul, T\u00fcrkiye will enable Turkish and international researchers to easily perform high- resolution structural analysis of biomacromolecules from single crystals. Here, we describe the technical and practical outlook of a state-of-the-art home-source X-ray, using lysozyme as a model protein. The methods and practice described in this article can be applied to any biological sample for structural studies. Therefore, this article will be a valuable practical guide from sample preparation to data analysis.<\/p>\n

 <\/p>\n

Keywords<\/strong>: X-ray crystallography; structural biology; atomic structures<\/p>\n","protected":false},"excerpt":{"rendered":"

Cahine Kulakman1, Hasan DeMirci1,2   1Department of Molecular Biology and Genetics, Koc University, 34450, Istanbul, Turkey 2Stanford PULSE Institute, SLAC National Laboratory, 94025, Menlo Park, CA, USA ckulakman22@ku.edu.tr   X-ray crystallography is a robust and powerful structural biology technique that provides high-resolution atomic structures of biomacromolecules. Scientists use this technique to unravel mechanistic and structural […]<\/p>\n","protected":false},"author":5,"featured_media":0,"parent":2553,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"ngg_post_thumbnail":0,"footnotes":""},"class_list":["post-2609","page","type-page","status-publish","hentry"],"aioseo_notices":[],"aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t