{"id":2609,"date":"2023-04-04T13:04:03","date_gmt":"2023-04-04T13:04:03","guid":{"rendered":"http:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/?page_id=2609"},"modified":"2023-04-04T13:04:03","modified_gmt":"2023-04-04T13:04:03","slug":"a11-cryogenic-x-ray-crystallographic-studies-of-biomacromolecules-at-turkish-light-source-turkish-delight","status":"publish","type":"page","link":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/abstracts\/a11-cryogenic-x-ray-crystallographic-studies-of-biomacromolecules-at-turkish-light-source-turkish-delight\/","title":{"rendered":"A11: Cryogenic X-ray crystallographic studies of biomacromolecules at Turkish Light Source \u201cTurkish DeLight&#8221;"},"content":{"rendered":"<p style=\"text-align: center;\"><strong>Cahine Kulakman<\/strong><sup>1<\/sup>, Hasan DeMirci<sup>1,2<\/sup><\/p>\n<p>&nbsp;<\/p>\n<p><sup>1<\/sup>Department of Molecular Biology and Genetics, Koc University, 34450, Istanbul, Turkey<br \/>\n<sup>2<\/sup>Stanford PULSE Institute, SLAC National Laboratory, 94025, Menlo Park, CA, USA<\/p>\n<p><a href=\"mailto:ckulakman22@ku.edu.tr\">ckulakman22@ku.edu.tr<\/a><\/p>\n<p>&nbsp;<\/p>\n<p style=\"text-align: justify;\">X-ray crystallography is a robust and powerful structural biology technique that provides high-resolution atomic structures of biomacromolecules. Scientists use this technique to unravel mechanistic and structural details of biological macromolecules (e.g. proteins, nucleic acids, protein complexes, protein-nucleic acid complexes, or large biological compartments). Since its inception, single-crystal cryo-crystallography has never been performed in T\u00fcrkiye due to the lack of a single-crystal X-ray diffractometer. The X-ray diffraction facility recently established at the University of Health Sciences, Istanbul, T\u00fcrkiye will enable Turkish and international researchers to easily perform high- resolution structural analysis of biomacromolecules from single crystals. Here, we describe the technical and practical outlook of a state-of-the-art home-source X-ray, using lysozyme as a model protein. The methods and practice described in this article can be applied to any biological sample for structural studies. Therefore, this article will be a valuable practical guide from sample preparation to data analysis.<\/p>\n<p style=\"text-align: justify;\">&nbsp;<\/p>\n<p style=\"text-align: justify;\"><strong>Keywords<\/strong>:&nbsp;X-ray crystallography; structural biology; atomic structures<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Cahine Kulakman1, Hasan DeMirci1,2 &nbsp; 1Department of Molecular Biology and Genetics, Koc University, 34450, Istanbul, Turkey 2Stanford PULSE Institute, SLAC National Laboratory, 94025, Menlo Park, CA, USA ckulakman22@ku.edu.tr &nbsp; X-ray crystallography is a robust and powerful structural biology technique that provides high-resolution atomic structures of biomacromolecules. Scientists use this technique to unravel mechanistic and structural [&hellip;]<\/p>\n","protected":false},"author":5,"featured_media":0,"parent":2553,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"ngg_post_thumbnail":0,"footnotes":""},"class_list":["post-2609","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-json\/wp\/v2\/pages\/2609","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-json\/wp\/v2\/comments?post=2609"}],"version-history":[{"count":1,"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-json\/wp\/v2\/pages\/2609\/revisions"}],"predecessor-version":[{"id":2610,"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-json\/wp\/v2\/pages\/2609\/revisions\/2610"}],"up":[{"embeddable":true,"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-json\/wp\/v2\/pages\/2553"}],"wp:attachment":[{"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/wp-json\/wp\/v2\/media?parent=2609"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}