{"id":2636,"date":"2023-04-04T13:22:48","date_gmt":"2023-04-04T13:22:48","guid":{"rendered":"http:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/?page_id=2636"},"modified":"2023-04-04T13:22:48","modified_gmt":"2023-04-04T13:22:48","slug":"a19-the-time-resolved-binding-of-initiation-factor-1-to-the-decoding-center-of-30s-ribosomal-subunit","status":"publish","type":"page","link":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc5\/abstracts\/a19-the-time-resolved-binding-of-initiation-factor-1-to-the-decoding-center-of-30s-ribosomal-subunit\/","title":{"rendered":"A19: The time-resolved binding of initiation factor 1 to the decoding center of 30S ribosomal subunit"},"content":{"rendered":"

Ilkin Yapici<\/strong>1<\/sup>, Hasan DeMirci1,2<\/sup>  <\/p>\n

 <\/sup><\/p>\n

1<\/sup>Department of Molecular Biology and Genetics, Koc University, 34450, Istanbul, Turkey
\n2<\/sup>Stanford PULSE Institute, SLAC National Laboratory, 94025, Menlo Park, CA, USA<\/p>\n

iyapici21@ku.edu.tr<\/a><\/p>\n

 <\/p>\n

Among the four phases of translation, initiation is the first, most rate-limiting, and most complicated translational phase. The binding of initiation factor 1 (IF-1) to the 30S ribosomal subunit is one of the most crucial steps to form a fully assembled translating ribosome, and its atomic details remain elusive. Thanks to X-ray Free Electron Lasers, we managed to observe the molecular details of protein-RNA and protein-protein interactions at atomic resolution in real-time. Our data illustrate the missing links between initial IF-1 interaction with critical decoding residues A1492 and A1493 from 16S RNA and enable us to observe conformational changes caused in the 30S subunit at hitherto time scales and resolution.<\/p>\n

 <\/p>\n

Keywords<\/strong>: structural dynamics; structural biology; crystallography; ribosome; time-resolved crystallography<\/p>\n","protected":false},"excerpt":{"rendered":"

Ilkin Yapici1, Hasan DeMirci1,2     1Department of Molecular Biology and Genetics, Koc University, 34450, Istanbul, Turkey 2Stanford PULSE Institute, SLAC National Laboratory, 94025, Menlo Park, CA, USA iyapici21@ku.edu.tr   Among the four phases of translation, initiation is the first, most rate-limiting, and most complicated translational phase. The binding of initiation factor 1 (IF-1) to the […]<\/p>\n","protected":false},"author":5,"featured_media":0,"parent":2553,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"ngg_post_thumbnail":0,"footnotes":""},"class_list":["post-2636","page","type-page","status-publish","hentry"],"aioseo_notices":[],"aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t