{"id":1605,"date":"2023-09-12T09:07:45","date_gmt":"2023-09-12T09:07:45","guid":{"rendered":"https:\/\/htcc6.org\/?page_id=1605"},"modified":"2023-09-15T06:47:51","modified_gmt":"2023-09-15T06:47:51","slug":"a1-bring-your-own-sample","status":"publish","type":"page","link":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc6\/a1-bring-your-own-sample\/","title":{"rendered":"A1: Bring your own sample"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"1605\" class=\"elementor elementor-1605\" data-elementor-post-type=\"page\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-646f43e elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"646f43e\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-05cb12d\" data-id=\"05cb12d\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-ac629a4 elementor-widget elementor-widget-text-editor\" data-id=\"ac629a4\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ul>\n<li>Having trouble crystallizing your protein\/nucleic acid\/complex?<\/li>\n<li>Getting crystals, but they don\u2019t diffract well? Or do you require a different morphology or space group?<\/li>\n<\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-e6433c9 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"e6433c9\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-86e5044\" data-id=\"86e5044\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-8b318ea elementor-widget elementor-widget-text-editor\" data-id=\"8b318ea\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\"><strong>YES &amp; YES?<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-d26efe1 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"d26efe1\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-ec22534\" data-id=\"ec22534\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-57d4e60 elementor-widget elementor-widget-text-editor\" data-id=\"57d4e60\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: justify;\">Then take part in the Activity A1: Bring your sample at the HTCC6, organized by Croatian Association of Crystallographers and Douglas Instruments! If you have a moment, complete the <a href=\"https:\/\/htcc6.org\/a1-online-form\/\">online form<\/a> before you come, or just bring your sample to the workshop.<\/p><p style=\"text-align: justify;\">As for the sample, we\u2019ll need roughly 10 &#8211; 60 \u00b5L of solution, usually at around 10 mg\/mL (although the best concentration depends on the solubility of the sample). If you happen to already have those poor crystals, and\/or crystals of a structurally related macromolecule, just bring them along, that\u2019d be awesome. If not, bring the solution, we\u2019ll manage.<\/p><p style=\"text-align: justify;\">Students will be taught how to make seed-stocks and given guidance for setting up crystallization experiments. They will also be shown how to set up microbatch-under-oil experiments, which are easier to scale up to make samples for serial data-collection, neutron diffraction, cryoEM<em> etc<\/em>.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-dbd6571 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"dbd6571\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-776b9bf\" data-id=\"776b9bf\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6861943 elementor-widget elementor-widget-image\" data-id=\"6861943\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc6\/wp-content\/uploads\/2023\/09\/Lecture-2-illustration-serial-data-collection-etc.png\" title=\"Lecture 2 illustration &#8211; serial data collection etc\" alt=\"Lecture 2 illustration - serial data collection etc\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-4b5f32c\" data-id=\"4b5f32c\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-9956814 elementor-widget elementor-widget-image\" data-id=\"9956814\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc6\/wp-content\/uploads\/2023\/09\/ConA-project-image.png\" title=\"ConA project image\" alt=\"ConA project image\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-9d5afe4 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"9d5afe4\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-36c3e1e\" data-id=\"36c3e1e\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-805da33 elementor-widget elementor-widget-text-editor\" data-id=\"805da33\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Important<\/strong>: We won\u2019t be able to fit in samples from all delegates so that the number will be limited to approximately 10 samples. \u00a0To guarantee your place, please complete and submit the online sample questionnaire.<\/p>\n<p>\u00a0<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-6c6e0f4 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"6c6e0f4\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-ff5673d\" data-id=\"ff5673d\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-81ce2c7 elementor-widget elementor-widget-text-editor\" data-id=\"81ce2c7\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>SESSIONS:<\/strong><\/p><ul><li><strong>07\/04\/2024, 21:00<\/strong>: Students who have brought samples should approach Douglas Instruments at the A1 desk to discuss their projects.<\/li><li><strong>08\/04\/2024, 21:00<\/strong>: Short theoretical introduction on the sample preparation for advanced methods of structural determination, followed by practical demonstration of how to make seed stock and set up microseeding experiments.<\/li><li><strong>10\/04\/2024, 14:00<\/strong>: Some of the new crystals obtained will be shown.<\/li><li><strong>11\/04\/2024, 18:30<\/strong>: Wrap-up (a short look back at the activity within the closing ceremony)<\/li><\/ul><p>\u00a0<\/p><p>The practical sessions will be coordinated by\u00a0<a href=\"https:\/\/www.douglas.co.uk\/\">Douglas Instruments<\/a>\u00a0who are kindly making equipment available for the workshop.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-91ec6b9 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"91ec6b9\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-3681104\" data-id=\"3681104\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-697b5ed elementor-widget elementor-widget-text-editor\" data-id=\"697b5ed\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>REFERENCES:<\/strong><\/p>\n<p>[1] D\u2019Arcy, Allan, Frederic Villard, and May Marsh. \u201cAn automated microseed matrix-screening method for protein crystallization.\u201d Acta Crystallographica Section D: Biological Crystallography 63.4\u00a0(2007): 550-554.<\/p>\n<p>[2] Shaw Stewart, Patrick D., et al. \u201cRandom microseeding: a theoretical and practical exploration of seed stability and seeding techniques for successful protein crystallization.\u201d Crystal Growth &amp;\u00a0Design\u00a011.8 (2011): 3432-3441.<\/p>\n<p>[3] Abuhammad, Areej, et al. \u201cStructure of arylamine N-acetyltransferase from Mycobacterium tuberculosis determined by cross-seeding with the homologous protein from M. marinum: triumph over adversity.\u201d\u00a0<em>Acta Crystallographica Section D: Biological Crystallography<\/em>\u00a069.8 (2013): 1433-1446.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Having trouble crystallizing your protein\/nucleic acid\/complex? Getting crystals, but they don\u2019t diffract well? Or do you require a different morphology or space group? YES &amp; YES? Then take part in the Activity A1: Bring your sample at the HTCC6, organized by Croatian Association of Crystallographers and Douglas Instruments! If you have a moment, complete the [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-1605","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc6\/wp-json\/wp\/v2\/pages\/1605","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc6\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc6\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc6\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc6\/wp-json\/wp\/v2\/comments?post=1605"}],"version-history":[{"count":33,"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc6\/wp-json\/wp\/v2\/pages\/1605\/revisions"}],"predecessor-version":[{"id":1725,"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc6\/wp-json\/wp\/v2\/pages\/1605\/revisions\/1725"}],"wp:attachment":[{"href":"https:\/\/hrvatska-udruga-kristalografa.hr\/htcc6\/wp-json\/wp\/v2\/media?parent=1605"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}